|
KMID : 0390320080180010314
|
|
Chungbuk Medical Journal
2008 Volume.18 No. 1 p.314 ~ p.321
|
|
|
Enhancement of thermal sensitivity by troglitazone in cervix cancer cells
|
|
Jeon Ha-Yeun
Park Yoon-Woo
|
|
|
Abstract
|
|
|
Purpose: Uterine cervix cancer is commonly treated with radiation therapy and the treatment results can be enhanced by combined with hyperthermia. Hyperthermia is more effective on the cells under the state of hypoxia and low pH. Troglitazone decreases intracellular pH and HSP(heat shock protein) 70 production and this may cause cells more sensitive to hyperthermia. Thus, we investigated the cytotoxic effect by the combination of hyperthermia and troglitazone in uterine cervix cancer cells.
Materials & Method: HeLa cells were treated with troglitazone(10 μM for 72 hr) alone, hyperthermia(42℃ for 1 hr) alone, or both(hyperthermia and troglitazone treatment). The cytotoxic effect was measured using clonogenic assay. The change of Hsp 70 expression was studied by western blot.
Results: Hsp 70 expression was induced from 24 hrs after hyperthermia. When the cells were preincubated with 10 μM of troglitazone for 72 hr Hsp 70 was not induced. The surviving fractions(SF) were 0.77 and 0.32, respectively for troglitazone alone and hyperthermia alone. When the cells were treated with troglitazone before hyperthermia the SF was 0.34.
Conclusions: Troglitazone prevents the induction of HSP 70 by hyperthermia. However, troglitazone itself does not increase thermal sensitivity in HeLa.
|
|
|
KEYWORD
|
|
|
cervix cancer, troglitazone, hyperthermia
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|
|